DNA

Part:BBa_J100140:Design

Designed by: Catherine Doyle   Group: Campbell M Lab   (2014-04-17)


Theophylline Riboswitch 4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3083
    Illegal suffix found in sequence at 877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3083
    Illegal SpeI site found at 878
    Illegal PstI site found at 892
    Illegal NotI site found at 885
    Illegal NotI site found at 3089
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3083
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3083
    Illegal suffix found in sequence at 878
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3083
    Illegal XbaI site found at 3098
    Illegal SpeI site found at 878
    Illegal PstI site found at 892
    Illegal AgeI site found at 100
    Illegal AgeI site found at 181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 169


Design Notes

no


Source

no

References